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recombinant ctrp3 chimerigen  (Chimerigen Laboratories)

 
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    Chimerigen Laboratories recombinant ctrp3 chimerigen
    Primers used in qRT-PCR .
    Recombinant Ctrp3 Chimerigen, supplied by Chimerigen Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+ctrp3+chimerigen/recombinant+ctrp3/pmc05069420-49-21-26
    Average 90 stars, based on 1 article reviews
    recombinant ctrp3 chimerigen - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat"

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2016.00237

    Primers used in qRT-PCR .
    Figure Legend Snippet: Primers used in qRT-PCR .

    Techniques Used:

    Effects of CTRP3 treatment on brain water content 7 days after ICH. (A) Brain water content in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with rCTRP3. (B) Brain water content in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with Lenti-CTRP3. Values are mean ± SEM. n = 8 per group. * p < 0.05 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti.CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.
    Figure Legend Snippet: Effects of CTRP3 treatment on brain water content 7 days after ICH. (A) Brain water content in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with rCTRP3. (B) Brain water content in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with Lenti-CTRP3. Values are mean ± SEM. n = 8 per group. * p < 0.05 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti.CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Techniques Used: Recombinant, Over Expression

    Effects of CTRP3 treatment on neurological functions 7 days after ICH. All animals after ICH showed significant neurological deficits based on performance on the modified Garcia (A) , beam balance (B) and wire hanging (C) tests. Rats treated with rCTRP3 showed reduced neurological deficits in all three tests. Treatment with Lenti-CTRP3 starting 2 weeks before induction of ICH showed a tendency to ameliorate neurological deficits (D–F) . Values are mean ± SEM. n = 9 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.
    Figure Legend Snippet: Effects of CTRP3 treatment on neurological functions 7 days after ICH. All animals after ICH showed significant neurological deficits based on performance on the modified Garcia (A) , beam balance (B) and wire hanging (C) tests. Rats treated with rCTRP3 showed reduced neurological deficits in all three tests. Treatment with Lenti-CTRP3 starting 2 weeks before induction of ICH showed a tendency to ameliorate neurological deficits (D–F) . Values are mean ± SEM. n = 9 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Techniques Used: Modification, Recombinant, Over Expression

    Effects of CTRP3 treatment on ICH-induced blood-brain barrier (BBB) damage 7 days after ICH. (A) Quantification of Evans blue dye extravasation (blue staining) in the ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with rCTRP3. (B) Quantification of Evans blue dye extravasation (blue staining) in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with Lenti-CTRP3. Values are mean ± SEM. n = 6 per group. ** p < 0.01 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.
    Figure Legend Snippet: Effects of CTRP3 treatment on ICH-induced blood-brain barrier (BBB) damage 7 days after ICH. (A) Quantification of Evans blue dye extravasation (blue staining) in the ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with rCTRP3. (B) Quantification of Evans blue dye extravasation (blue staining) in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with Lenti-CTRP3. Values are mean ± SEM. n = 6 per group. ** p < 0.01 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Techniques Used: Staining, Recombinant, Over Expression

    CTRP3 encourages angiogenesis in the hematoma border zone 7 days after ICH. (A) Capillary density computed by immunohistochemical staining for CD31 (brown) in the perifocal region in ICH (b) , rCTRP3 (c) and Lenti-CTRP3 (d) 7 days after ICH. A control brain section demonstrates a normal distribution of microvessels in the same region (a) . Bar = 100 μm. (B) Bar graph showing the number of brown stained capillaries 7 days after ICH. Values are mean ± SEM. n = 5 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; # p < 0.05 vs. ICH. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage; CD31, platelet endothelial cell adhesion molecule-1.
    Figure Legend Snippet: CTRP3 encourages angiogenesis in the hematoma border zone 7 days after ICH. (A) Capillary density computed by immunohistochemical staining for CD31 (brown) in the perifocal region in ICH (b) , rCTRP3 (c) and Lenti-CTRP3 (d) 7 days after ICH. A control brain section demonstrates a normal distribution of microvessels in the same region (a) . Bar = 100 μm. (B) Bar graph showing the number of brown stained capillaries 7 days after ICH. Values are mean ± SEM. n = 5 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; # p < 0.05 vs. ICH. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage; CD31, platelet endothelial cell adhesion molecule-1.

    Techniques Used: Immunohistochemical staining, Staining, Control, Recombinant, Over Expression

    CTRP3 upregulates pAMPK/HIF-1α/vascular endothelial growth factor (VEGF) axis protein expression in the hematoma border zone 7 days after ICH. (A) Western blot analysis of the effect of rCTRP3 on CTRP3, phosphorylated (p) AMP-activated protein kinase (AMPK; Thr172)/AMPK, HIF-1α and VEGF-A. (B–E) Representative ratios of CTRP3, p-AMPK, HIF-1α and VEGF-A protein expression. (F) Western blot analysis of the effect of Lenti-CTRP3 on CTRP3, phosphorylated (p) AMPK (Thr172)/AMPK, HIF-1α and VEGF-A. (G–J) Representative ratios of CTRP3, p-AMPK, HIF-1α and VEGF-A protein expression. Values are mean ± SEM. n = 4 per group. * p < 0.05 vs. sham; ## p < 0.01 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.
    Figure Legend Snippet: CTRP3 upregulates pAMPK/HIF-1α/vascular endothelial growth factor (VEGF) axis protein expression in the hematoma border zone 7 days after ICH. (A) Western blot analysis of the effect of rCTRP3 on CTRP3, phosphorylated (p) AMP-activated protein kinase (AMPK; Thr172)/AMPK, HIF-1α and VEGF-A. (B–E) Representative ratios of CTRP3, p-AMPK, HIF-1α and VEGF-A protein expression. (F) Western blot analysis of the effect of Lenti-CTRP3 on CTRP3, phosphorylated (p) AMPK (Thr172)/AMPK, HIF-1α and VEGF-A. (G–J) Representative ratios of CTRP3, p-AMPK, HIF-1α and VEGF-A protein expression. Values are mean ± SEM. n = 4 per group. * p < 0.05 vs. sham; ## p < 0.01 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Techniques Used: Expressing, Western Blot, Recombinant, Over Expression

    Quantitative RT-PCR analysis of HIF-1α and VEGF-A mRNA levels in the hematoma border zone 7 days after ICH. (A–C) Quantitative RT-PCR analysis of the effect of rCTRP3 on CTRP3, HIF-1α and VEGF-A mRNA levels. (D–F) Quantitative RT-PCR analysis of the effect of rCTRP3 on CTRP3, HIF-1α and VEGF-A mRNA levels. Values are mean ± SEM. n = 4–6 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; ## p < 0.01 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti.CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.
    Figure Legend Snippet: Quantitative RT-PCR analysis of HIF-1α and VEGF-A mRNA levels in the hematoma border zone 7 days after ICH. (A–C) Quantitative RT-PCR analysis of the effect of rCTRP3 on CTRP3, HIF-1α and VEGF-A mRNA levels. (D–F) Quantitative RT-PCR analysis of the effect of rCTRP3 on CTRP3, HIF-1α and VEGF-A mRNA levels. Values are mean ± SEM. n = 4–6 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; ## p < 0.01 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti.CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Techniques Used: Quantitative RT-PCR, Recombinant, Over Expression

    CTRP3 promotes angiogenesis via an AMPK-dependent signaling mechanism. (A) Western blot analysis of the effects of COM.C and CBO on rCTRP3-induced p-AMPK, HIF-1α and VEGF-A expression. (B–D) Representative ratios of p-AMPK, HIF-1α and VEGF-A protein expression. (E) Modified Garcia test analysis of the effects of COM.C and CBO on neurological function improvement induced by rCTRP3. (F) Evans blue dye extravasation analysis of the effects of COM.C and CBO on ICH-induced BBB damage reduced by rCTRP3. (G) Immunohistochemical staining analysis of the effects of COM.C and CBO on angiogenesis induced by rCTRP3. Capillary density measured by immunohistochemical staining for CD31 (brown) in the perifocal region in ICH (a) , rCTRP3 (b) , COM.C (c) and CBO (d) 7 days after ICH. (H) Bar graph showing the number of brown stained capillaries. Values are mean ± SEM. n = 4–6 per group. ** p < 0.01 vs. ICH; # p < 0.05 vs. rCTRP3; ## p < 0.01 vs. rCTRP3; & p < 0.05 vs. COM.C. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage; COM.C, compound C; CBO, CBO-P11.
    Figure Legend Snippet: CTRP3 promotes angiogenesis via an AMPK-dependent signaling mechanism. (A) Western blot analysis of the effects of COM.C and CBO on rCTRP3-induced p-AMPK, HIF-1α and VEGF-A expression. (B–D) Representative ratios of p-AMPK, HIF-1α and VEGF-A protein expression. (E) Modified Garcia test analysis of the effects of COM.C and CBO on neurological function improvement induced by rCTRP3. (F) Evans blue dye extravasation analysis of the effects of COM.C and CBO on ICH-induced BBB damage reduced by rCTRP3. (G) Immunohistochemical staining analysis of the effects of COM.C and CBO on angiogenesis induced by rCTRP3. Capillary density measured by immunohistochemical staining for CD31 (brown) in the perifocal region in ICH (a) , rCTRP3 (b) , COM.C (c) and CBO (d) 7 days after ICH. (H) Bar graph showing the number of brown stained capillaries. Values are mean ± SEM. n = 4–6 per group. ** p < 0.01 vs. ICH; # p < 0.05 vs. rCTRP3; ## p < 0.01 vs. rCTRP3; & p < 0.05 vs. COM.C. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage; COM.C, compound C; CBO, CBO-P11.

    Techniques Used: Western Blot, Expressing, Modification, Immunohistochemical staining, Staining, Recombinant, Over Expression

    Related Articles

    Recombinant:

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat
    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Injection:

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat
    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Over Expression:

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat
    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Modification:

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat
    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Staining:

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat
    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Immunohistochemical staining:

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat
    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Control:

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat
    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Expressing:

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat
    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Western Blot:

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat
    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Quantitative RT-PCR:

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat
    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.



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    recombinant ctrp3 chimerigen  (Chimerigen Laboratories)
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    Chimerigen Laboratories recombinant ctrp3 chimerigen
    Primers used in qRT-PCR .
    Recombinant Ctrp3 Chimerigen, supplied by Chimerigen Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+ctrp3+chimerigen/recombinant+ctrp3/pmc05069420-49-21-26
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    		  Buy from Supplier

    Image Search Results


    Primers used in qRT-PCR .

    Journal: Frontiers in Cellular Neuroscience

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat

    doi: 10.3389/fncel.2016.00237

    Figure Lengend Snippet: Primers used in qRT-PCR .

    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Techniques:

    Effects of CTRP3 treatment on brain water content 7 days after ICH. (A) Brain water content in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with rCTRP3. (B) Brain water content in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with Lenti-CTRP3. Values are mean ± SEM. n = 8 per group. * p < 0.05 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti.CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat

    doi: 10.3389/fncel.2016.00237

    Figure Lengend Snippet: Effects of CTRP3 treatment on brain water content 7 days after ICH. (A) Brain water content in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with rCTRP3. (B) Brain water content in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with Lenti-CTRP3. Values are mean ± SEM. n = 8 per group. * p < 0.05 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti.CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Techniques: Recombinant, Over Expression

    Effects of CTRP3 treatment on neurological functions 7 days after ICH. All animals after ICH showed significant neurological deficits based on performance on the modified Garcia (A) , beam balance (B) and wire hanging (C) tests. Rats treated with rCTRP3 showed reduced neurological deficits in all three tests. Treatment with Lenti-CTRP3 starting 2 weeks before induction of ICH showed a tendency to ameliorate neurological deficits (D–F) . Values are mean ± SEM. n = 9 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat

    doi: 10.3389/fncel.2016.00237

    Figure Lengend Snippet: Effects of CTRP3 treatment on neurological functions 7 days after ICH. All animals after ICH showed significant neurological deficits based on performance on the modified Garcia (A) , beam balance (B) and wire hanging (C) tests. Rats treated with rCTRP3 showed reduced neurological deficits in all three tests. Treatment with Lenti-CTRP3 starting 2 weeks before induction of ICH showed a tendency to ameliorate neurological deficits (D–F) . Values are mean ± SEM. n = 9 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Techniques: Modification, Recombinant, Over Expression

    Effects of CTRP3 treatment on ICH-induced blood-brain barrier (BBB) damage 7 days after ICH. (A) Quantification of Evans blue dye extravasation (blue staining) in the ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with rCTRP3. (B) Quantification of Evans blue dye extravasation (blue staining) in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with Lenti-CTRP3. Values are mean ± SEM. n = 6 per group. ** p < 0.01 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat

    doi: 10.3389/fncel.2016.00237

    Figure Lengend Snippet: Effects of CTRP3 treatment on ICH-induced blood-brain barrier (BBB) damage 7 days after ICH. (A) Quantification of Evans blue dye extravasation (blue staining) in the ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with rCTRP3. (B) Quantification of Evans blue dye extravasation (blue staining) in ipsilateral (Ipsi) and contralateral (Contra) hemispheres 7 days after ICH in rats treated with Lenti-CTRP3. Values are mean ± SEM. n = 6 per group. ** p < 0.01 vs. sham; # p < 0.05 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Techniques: Staining, Recombinant, Over Expression

    CTRP3 encourages angiogenesis in the hematoma border zone 7 days after ICH. (A) Capillary density computed by immunohistochemical staining for CD31 (brown) in the perifocal region in ICH (b) , rCTRP3 (c) and Lenti-CTRP3 (d) 7 days after ICH. A control brain section demonstrates a normal distribution of microvessels in the same region (a) . Bar = 100 μm. (B) Bar graph showing the number of brown stained capillaries 7 days after ICH. Values are mean ± SEM. n = 5 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; # p < 0.05 vs. ICH. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage; CD31, platelet endothelial cell adhesion molecule-1.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat

    doi: 10.3389/fncel.2016.00237

    Figure Lengend Snippet: CTRP3 encourages angiogenesis in the hematoma border zone 7 days after ICH. (A) Capillary density computed by immunohistochemical staining for CD31 (brown) in the perifocal region in ICH (b) , rCTRP3 (c) and Lenti-CTRP3 (d) 7 days after ICH. A control brain section demonstrates a normal distribution of microvessels in the same region (a) . Bar = 100 μm. (B) Bar graph showing the number of brown stained capillaries 7 days after ICH. Values are mean ± SEM. n = 5 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; # p < 0.05 vs. ICH. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage; CD31, platelet endothelial cell adhesion molecule-1.

    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Techniques: Immunohistochemical staining, Staining, Control, Recombinant, Over Expression

    CTRP3 upregulates pAMPK/HIF-1α/vascular endothelial growth factor (VEGF) axis protein expression in the hematoma border zone 7 days after ICH. (A) Western blot analysis of the effect of rCTRP3 on CTRP3, phosphorylated (p) AMP-activated protein kinase (AMPK; Thr172)/AMPK, HIF-1α and VEGF-A. (B–E) Representative ratios of CTRP3, p-AMPK, HIF-1α and VEGF-A protein expression. (F) Western blot analysis of the effect of Lenti-CTRP3 on CTRP3, phosphorylated (p) AMPK (Thr172)/AMPK, HIF-1α and VEGF-A. (G–J) Representative ratios of CTRP3, p-AMPK, HIF-1α and VEGF-A protein expression. Values are mean ± SEM. n = 4 per group. * p < 0.05 vs. sham; ## p < 0.01 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat

    doi: 10.3389/fncel.2016.00237

    Figure Lengend Snippet: CTRP3 upregulates pAMPK/HIF-1α/vascular endothelial growth factor (VEGF) axis protein expression in the hematoma border zone 7 days after ICH. (A) Western blot analysis of the effect of rCTRP3 on CTRP3, phosphorylated (p) AMP-activated protein kinase (AMPK; Thr172)/AMPK, HIF-1α and VEGF-A. (B–E) Representative ratios of CTRP3, p-AMPK, HIF-1α and VEGF-A protein expression. (F) Western blot analysis of the effect of Lenti-CTRP3 on CTRP3, phosphorylated (p) AMPK (Thr172)/AMPK, HIF-1α and VEGF-A. (G–J) Representative ratios of CTRP3, p-AMPK, HIF-1α and VEGF-A protein expression. Values are mean ± SEM. n = 4 per group. * p < 0.05 vs. sham; ## p < 0.01 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Techniques: Expressing, Western Blot, Recombinant, Over Expression

    Quantitative RT-PCR analysis of HIF-1α and VEGF-A mRNA levels in the hematoma border zone 7 days after ICH. (A–C) Quantitative RT-PCR analysis of the effect of rCTRP3 on CTRP3, HIF-1α and VEGF-A mRNA levels. (D–F) Quantitative RT-PCR analysis of the effect of rCTRP3 on CTRP3, HIF-1α and VEGF-A mRNA levels. Values are mean ± SEM. n = 4–6 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; ## p < 0.01 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti.CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat

    doi: 10.3389/fncel.2016.00237

    Figure Lengend Snippet: Quantitative RT-PCR analysis of HIF-1α and VEGF-A mRNA levels in the hematoma border zone 7 days after ICH. (A–C) Quantitative RT-PCR analysis of the effect of rCTRP3 on CTRP3, HIF-1α and VEGF-A mRNA levels. (D–F) Quantitative RT-PCR analysis of the effect of rCTRP3 on CTRP3, HIF-1α and VEGF-A mRNA levels. Values are mean ± SEM. n = 4–6 per group. * p < 0.05 vs. sham; ** p < 0.01 vs. sham; ## p < 0.01 vs. vehicle or Lenti.null. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti.CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage.

    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Techniques: Quantitative RT-PCR, Recombinant, Over Expression

    CTRP3 promotes angiogenesis via an AMPK-dependent signaling mechanism. (A) Western blot analysis of the effects of COM.C and CBO on rCTRP3-induced p-AMPK, HIF-1α and VEGF-A expression. (B–D) Representative ratios of p-AMPK, HIF-1α and VEGF-A protein expression. (E) Modified Garcia test analysis of the effects of COM.C and CBO on neurological function improvement induced by rCTRP3. (F) Evans blue dye extravasation analysis of the effects of COM.C and CBO on ICH-induced BBB damage reduced by rCTRP3. (G) Immunohistochemical staining analysis of the effects of COM.C and CBO on angiogenesis induced by rCTRP3. Capillary density measured by immunohistochemical staining for CD31 (brown) in the perifocal region in ICH (a) , rCTRP3 (b) , COM.C (c) and CBO (d) 7 days after ICH. (H) Bar graph showing the number of brown stained capillaries. Values are mean ± SEM. n = 4–6 per group. ** p < 0.01 vs. ICH; # p < 0.05 vs. rCTRP3; ## p < 0.01 vs. rCTRP3; & p < 0.05 vs. COM.C. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage; COM.C, compound C; CBO, CBO-P11.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: C1q/Tumor Necrosis Factor-Related Protein-3 Attenuates Brain Injury after Intracerebral Hemorrhage via AMPK-Dependent Pathway in Rat

    doi: 10.3389/fncel.2016.00237

    Figure Lengend Snippet: CTRP3 promotes angiogenesis via an AMPK-dependent signaling mechanism. (A) Western blot analysis of the effects of COM.C and CBO on rCTRP3-induced p-AMPK, HIF-1α and VEGF-A expression. (B–D) Representative ratios of p-AMPK, HIF-1α and VEGF-A protein expression. (E) Modified Garcia test analysis of the effects of COM.C and CBO on neurological function improvement induced by rCTRP3. (F) Evans blue dye extravasation analysis of the effects of COM.C and CBO on ICH-induced BBB damage reduced by rCTRP3. (G) Immunohistochemical staining analysis of the effects of COM.C and CBO on angiogenesis induced by rCTRP3. Capillary density measured by immunohistochemical staining for CD31 (brown) in the perifocal region in ICH (a) , rCTRP3 (b) , COM.C (c) and CBO (d) 7 days after ICH. (H) Bar graph showing the number of brown stained capillaries. Values are mean ± SEM. n = 4–6 per group. ** p < 0.01 vs. ICH; # p < 0.05 vs. rCTRP3; ## p < 0.01 vs. rCTRP3; & p < 0.05 vs. COM.C. CTRP3, C1q/tumor necrosis factor-related protein-3; rCTRP3, recombinant CTRP3; Lenti-CTRP3, Lentivirus overexpression of CTRP3; ICH, intracerebral hemorrhage; COM.C, compound C; CBO, CBO-P11.

    Article Snippet: Adult rats were split at random into the following four groups: sham-operated (sham) group, ICH group, ICH + vehicle group and ICH + recombinant CTRP3 (rCTRP3, Chimerigen, USA) group. rCTRP3 was injected intracerebroventricularly (80 μg/kg) 30 min after ICH and then three times per week until the animals were killed.

    Techniques: Western Blot, Expressing, Modification, Immunohistochemical staining, Staining, Recombinant, Over Expression